Selection of membrane depends on the assay. Ag-Ab interaction during actual assay depends upon binding strength of nitrocellulose membrane. The selection of an appropriate nitrocellulose membrane is pivotal for the development of a high performance lateral flow test. Each type of nitrocellulose membrane has unique capillary flow rate based on the physical attributes of the membrane and the manufacturing process that impact the flow dynamics, sensitivity, specificity, and consistency of the assay